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目的评价不同批次虎杖饮片一致性与差异性,为其质量控制提供方法参考。方法采用HPLC法建立11批虎杖饮片的化学指纹图谱;采用总量统计矩法计算总量零阶矩(AUCT)、总量响应率(AUCPWT)、总量一阶矩(MCRTT)、总量二阶矩(VCRTT)等参数;采用总量统计矩法、夹角余弦法和相关系数法进行相似度评价;采用聚类分析法与主成分分析(PCA)法对虎杖样品进行分类分析。结果对11批虎杖饮片进行测定,共标定了13个共有峰,指认了虎杖苷、白藜芦醇、大黄素苷、大黄素的色谱峰;总量统计矩参数AUCT、AUCPWT、MCRTT、VCRTT的平均值分别为1.52×106 AU·min、2.31×103 AU·min·mL/mg、22.0 min、110.0 min2;各批次样品的相似度在0.85以上;聚类分析法与PCA法均将样品分为4类。结论化学指纹图谱结合总量统计矩分析、聚类分析与PCA可评价虎杖饮片一致性与差异性,是适合中药特点的质量控制方法。
Objective To evaluate the consistency and difference of different samples of Polygonum cuspidatum and to provide a reference for its quality control. Methods The chemical fingerprints of eleven batches of Polygonum cuspidatum were determined by HPLC method. The AUCT, AUCPWT, MCRTT, (VCRTT) and so on. The similarity of total statistical moment method, cosine angle method and correlation coefficient method were used to evaluate the similarity. Polygonum cuspidatum samples were classified by cluster analysis and principal component analysis (PCA). Results A total of 13 common peaks were identified on 11 batches of Polygonum cuspidatum Pieces. The peaks of polydatin, resveratrol, emodin and emodin were identified. The total statistical moment parameters AUCT, AUCPWT, MCRTT and VCRTT The average values were 1.52 × 106 AU · min, 2.31 × 103 AU · min · mL / mg, 22.0 min and 110.0 min2, respectively. The similarity of each sample was above 0.85. Both cluster analysis and PCA For 4 categories. Conclusion The chemical fingerprinting combined with the total amount of statistical moment analysis, cluster analysis and PCA can evaluate the concordance and difference of Polygonum cuspidatum Pieces, which is a quality control method suitable for traditional Chinese medicine.