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An attempt has been made of cryopreservation of tissue fragments from 6 GCTB surgicalspecimens. They have been kept in 10% DMSO or 15% glycerol medium and stored in liquidnitrogen for periods up to 180 days. Tumor cellular components survive the process of slowfreezing and rapid thawing as cellular growth is successful in subsequent culture. Thoughcertain reversible ultrastructural changes have been detected, no apparent distinction couldbe found in microscopic morphology, motility, growth behavior and LDH isozyme pattern be-tween cultured cells of the cryopreserved fragments and those of the fresh ones. Electronmicroscopy shows that DMSO exceeds glycerol in protecting the ultrastructure of the cells.Slow cooling and rapid thawing is recommended because ice-crystals form in the intercellularspace. which causes only light damage to the cells.
An attempt has been made of cryopreservation of tissue fragments from 6 GCTB surgicalspecimens. They have been kept in 10% DMSO or 15% glycerol medium and stored in liquidnitrogen for periods up to 180 days. Tumor cellular components survive the process of slowfreezing and rapid thawing as cellular growth is successful in subsequent culture. Though the apparent reversible ultrastructural changes have been detected, no apparent distinction could be found in microscopic morphology, motility, growth behavior and LDH isozyme pattern be-tween cultured cells of the cryopreserved fragments and those of the fresh ones. Electronmicroscopy shows that DMSO exceeds glycerol in protecting the ultrastructure of the cells. Cooling and rapid thawing is recommended because ice-crystals form in the intercellularspace. Which causes only light damage to the cells.