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目的:探讨肾素-血管紧张素系统基因中血管紧张素原(AGT)基因M235T多态、血管紧张素Ⅰ转换酶(ACE)基因插入/缺失(I/D)多态和血管紧张素Ⅱ-1型受体(AT1R)基因A1166C多态与子痫前期的关系。方法:应用聚合酶链反应(PCR)、限制性酶切及电泳分型等方法对45例子痫前期患者(子痫前期组)和45例非高血压妊娠妇女(对照组)的AGT基因M235T多态、ACE基因I/D多态、AT1R基因A1166C多态性进行分析。结果:(1)AGTM235T多态的TT基因频率两组比较差异有显著性。(2)ACE基因DD、ID、II型在两组中分布无明显差异。(3)AT1R基因A1166CAA、AC、CC型在两组中分布无明显差异。(4)子痫前期组具备TT基因型者,其合并DD基因型的95%CI为1.473～31.919,合并1166C等位基因C的95%CI为1.316～18.991。结论:AGT变异基因235T与子痫前期发生有关,T等位基因可能是子痫前期的易感基因。ACE基因DD型与子痫前期的相关性限于携带有M235T等位基因的患者。携带有AT1R基因C等位基因和AGT基因TT型的妇女子痫前期发病危险升高。
Objective: To investigate the polymorphisms of angiotensinogen (AGT) gene M235T, angiotensin I converting enzyme (ACE) gene insertion / deletion (I / D) polymorphism and angiotensin Ⅱ - Relationship between A1166C polymorphism of type 1 receptor (AT1R) gene and preeclampsia. Methods: The AGT gene M235T in 45 cases of preeclampsia (preeclampsia group) and 45 non-hypertensive pregnant women (control group) were detected by polymerase chain reaction (PCR), restriction enzyme digestion and electrophoresis. State, ACE gene I / D polymorphism and AT1R gene A1166C polymorphism were analyzed. Results: (1) The frequency of TT gene of AGTM235T polymorphism was significantly different between the two groups. (2) ACE gene DD, ID, II distribution in the two groups no significant difference. (3) The AT1R gene A1166CAA, AC, CC type distribution in the two groups no significant difference. (4) TT genotypes of preeclampsia group had 95% CI of 1.473 ~ 31.919 with combined DD genotype and 1.316 ~ 18.991 with 1166C allele. Conclusion: AGT variant gene 235T is associated with preeclampsia and T allele may be a susceptibility gene in preeclampsia. The association of ACE genotype DD with preeclampsia was limited to patients carrying the M235T allele. Women with the AT1R C allele and the AGT TT genotype have a higher risk of developing preeclampsia.