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目的 探讨PCNA基因过度表达在子宫颈上皮内瘤变(CIN)及子宫颈鳞形细胞癌中的意义。方法 标本采用常规石蜡切片、HE染色及ABC法免疫组化染色,光镜观察,并对增殖细胞核抗原指数(PCNALI)进行计数。结果 从正常宫颈鳞状上皮到CINⅡ级到子宫颈鳞癌,表达PCNA阳性的细胞范围和阳性强度呈明显增高的趋势,三者间PCNALI差异有显著性(P<001)。在45例鳞状细胞癌中PCNALI随着肿瘤分级增高而增高。鳞癌Ⅰ级、Ⅱ级和Ⅲ级之间PCNALI差异有显著性(P<005)。局部淋巴结有转移癌者和无转移癌者PCNALI差异亦有显著性(P<005)。结论 PCNALI对于检测宫颈鳞癌的发生与发展、早期诊断及判断肿瘤的生物学行为和预测患者的预后等方面具有重要的意义。
Objective To investigate the significance of PCNA gene overexpression in cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma. Methods The specimens were stained with paraffin, HE staining and ABC immunohistochemical staining, observed with light microscope, and counted the number of proliferating cell nuclear antigen (PCNALI). Results From normal cervical squamous epithelium to CINⅡgrade to cervical squamous cell carcinoma, the cell range and positive intensity of PCNA positive expression showed a significant increase trend, the difference of PCNALI was significant (P <001). In 45 cases of squamous cell carcinoma PCNALI increased with the tumor grade increased. There was significant difference in PCNALI between grade Ⅰ, grade Ⅱ and grade Ⅲ in squamous cell carcinoma (P <0.05). There was also significant difference in PCNALI between those with lymph node metastasis and without metastasis (P <005). Conclusion PCNALI plays an important role in detecting the occurrence and development of cervical squamous cell carcinoma, early diagnosis and judging the biological behavior of the tumor and prognosis of the patients.