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目的体外诱导细胞因子诱导的杀伤细胞(CIK),并研究其生物学活性。方法从外周血和脐血分离单个核细胞(PBMC),经过细胞因子诱导、培养并扩增CIK细胞,以LAK作比较,流式细胞仪检测CIK细胞表面标志CD3、CD56。3H-TdR释放法检测CIK的增殖能力,MTT法检测对肝癌细胞系SMMC-7721、Bel-7402,正常胎肝细胞L-02的杀伤活性。结果CIK细胞第二周进入快速增殖期,到第31d扩增倍数超过60倍,CD3+CD56+细胞扩增倍数达800倍以上;CIK对肝癌细胞的杀伤能力明显优于LAK细胞(65%～81%);对正常胎肝细胞的细胞毒作用(<5%)。结论CIK细胞是一种具有很强杀瘤活性的免疫活性细胞,具有临床应用前景。
Objective To induce cytokine-induced killer (CIK) cells in vitro and to study their biological activity. Methods Mononuclear cells (PBMCs) were isolated from peripheral blood and umbilical cord blood. After induced by cytokines, CIK cells were cultured and expanded. Compared with LAK, flow cytometry was used to detect CD3 and CD56.3H-TdR release of CIK cells The proliferation of CIK cells was detected by MTT assay. The cytotoxic activity of CIK on hepatoma cell lines SMMC-7721, Bel-7402 and normal fetal liver cells L-02 was detected by MTT assay. Results CIK cells entered the rapid proliferative phase in the second week, multiplied by more than 60 fold on the 31st day and multiplied more than 800 times on the CD3 + CD56 + cells. The cytotoxicity of CIK on hepatoma cells was significantly better than that of LAK cells (65% -81 %); Cytotoxic effect on normal fetal liver cells (<5%). Conclusion CIK cells are a kind of immunocompetent cells with strong antitumor activity and have clinical application prospect.