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通过对83016菌株生理生化特性、不同培养和发酵条件下制备物室内毒力测定方法比较及小试产品对松毛虫等5种森林害虫林间防治试验结果表明,该菌株属苏云金芽孢杆菌库尔斯塔克亚种;三种不同制备方法的室内生测比较,以光密度测定法较其它两种方法简便可靠,适用于进行大量菌株筛选;83016菌株发酵产物和小试产品对马尾松毛虫等害虫有较高的毒性。
Based on the physiological and biochemical characteristics of 83016 strain, comparison of indoor toxicity determination methods of preparations under different culture and fermentation conditions, and the experiment of prevention and control of five kinds of forest pests such as Dendrolimus punctatus, the results showed that the strain belongs to Bacillus thuringiensis Coles Tucker species; Comparison of three different preparation methods for indoor bioassay, compared with the other two methods of densitometry is simple and reliable, suitable for screening a large number of strains; 83016 strains of fermentation products and test products on Dendrolimus punctatus and other pests High toxicity.