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[目的]为了建立快速测定甘蓝类蔬菜(甘蓝、青花菜、芥蓝、苤蓝)中莱菔硫烷活性成分的方法。[方法]采用超高效液相色谱-串联质谱UPLC-MS/MS,快速测定甘蓝类蔬菜中莱菔硫烷含量。[结果]采用酶解-乙酸乙酯萃取法提取莱菔硫烷,Waters BEH反相C18(2.1 mm×50.0 mm,1.7μm)分离,质谱采用电喷雾正离子ESI(+)电离,多反应监测扫描模式(MRM)检测。在4种甘蓝类蔬菜(可食性器官)中均检测到莱菔硫烷成分,在0.1~20.0μg/L范围内线性关系良好,相关系数为0.999 4,相对标准偏差(RSD,n=6)为2.4%,检出限(LOD,S/N=3)为0.06~0.15μg/L,定量下限(LOQ,S/N=10)为0.3~0.4μg/L。[结论]该方法准确可靠,能够快速检测甘蓝类蔬菜中莱菔硫烷抗癌活性成分。
[Objective] The research aimed to establish a method for rapid determination of sulforaphane in cabbage vegetables (cabbage, broccoli, kale, and blueberry). [Method] The content of sulforaphane in cabbage vegetables was rapidly determined by UPLC-MS / MS. [Result] Sulforaphane was extracted by enzymatic hydrolysis - ethyl acetate extraction and separated on a Waters BEH reversed - phase C18 (2.1 mm × 50.0 mm, 1.7 μm). The mass spectra were determined by electrospray ionization ESI (+) ionization and multiple reaction monitoring Mode (MRM) test. Sulforaphane was detected in four kinds of cabbage vegetables (edible organs) with a good linearity in the range of 0.1-20.0 μg / L with a correlation coefficient of 0.999 4. The relative standard deviations (RSD, n = 6) were The detection limit (LOD, S / N = 3) was 0.06 ~ 0.15μg / L, and the lower limit of quantitation (LOQ, S / N = 10) was 0.3 ~ 0.4μg / L. [Conclusion] The method was accurate and reliable, and could rapidly detect the anti-cancer active ingredients of sulforaphane in cabbage vegetable.