目的　建立丹参三七配伍水煎液中丹酚酸B含量测定的方法。方法　大连依利特SpherisorbC18柱 ,流动相 :甲醇 -水 (用甲酸调pH =2 .5 ) ,体积比甲醇 -水 (4 1 5∶ 5 8 5 ) ,流速 :0 8mL/min ,检测波长 :2 80nm ,柱温 :2 5℃。结果　丹参∶三七配比 5∶ 3时 ,合煎或单煎对丹酚酸B溶出影响不大。结论　用此方法可以准确测定丹参三七配伍水煎液中丹酚酸B的含量 Objective To establish a method for the determination of salvianolic acid B in the decoction of Radix Salviae Miltiorrhizae. METHODS: Dalian ELITE Spherisorb C18 column, mobile phase: methanol-water (pH adjusted to 2.5 with formic acid), volume ratio of methanol-water (4 1 5: 5 8 5), flow rate: 0 8 mL/min, detection wavelength: 2 80nm, column temperature: 2 5°C. Results Salvia miltiorrhiza: Panax notoginseng ratio 5: 3, combined decoction or single decoction had little effect on salvianolic acid B dissolution. Conclusion This method can accurately determine the content of salvianolic acid B in the decoction of Radix Salviae Miltiorrhizae Mixture