论文部分内容阅读
目的:研究不同生物反应器对丹参不定根培养的影响。方法:利用组织培养技术结合高效液相色谱法,考察不同生物反应器对丹参不定根生长以及丹参不定根有效成分丹参酮ⅡA(TA)及原儿茶醛(PA)含量积累的影响。结果:不同反应器培养丹参不定根中,60°圆锥型反应器最有利于丹参不定根有效成分的积累。测定60°圆锥型反应器中的丹参不定根生长曲线以及有效成分产物积累曲线形如“S”型。丹参不定根培养在第35天达到最大生物量为16.24 g.L-1(鲜重),有效成分丹参酮ⅡA(TA)和原儿茶醛(PA)在第40天达到最高,分别为0.23,0.51 mg.g-1干重。结论:60°圆锥型反应器最有利于丹参不定根有效成分的积累,本实验对于丹参不定根大规模培养具有潜在意义。
Objective: To study the effect of different bioreactors on adventitious root culture of Salvia miltiorrhiza. Methods: The effects of different bioreactors on the growth of adventitious roots of Salvia miltiorrhiza and the accumulation of tanshinone IIA (TA) and protocatechuic aldehyde (PA) in the adventitious roots of Salvia miltiorrhiza were studied by tissue culture and high performance liquid chromatography. Results: In the adventitious roots of Salvia miltiorrhiza cultured in different reactors, the 60 ° conical reactor was the most favorable for the accumulation of active components in adventitious roots of Salvia miltiorrhiza. The growth curve of adventitious roots of Salvia miltiorrhiza in a 60° cone-type reactor and the accumulation curve of the active ingredient product were measured as a “S” shape. Adventitious root culture of Salvia miltiorrhiza reached the maximum biomass of 16.24 gL-1 (fresh weight) on the 35th day, and the active ingredients tanshinone IIA (TA) and protocatechuic aldehyde (PA) reached the highest on the 40th day, 0.23 and 0.51 mg, respectively. G-1 dry weight. Conclusion: The 60° conical reactor is most beneficial to the accumulation of effective components of adventitious roots of Salvia miltiorrhiza. This experiment has potential significance for the large-scale culture of Salvia miltiorrhiza adventitious roots.