[目的]研究鳖甲煎丸含药大鼠血清对人肝癌HEPG2细胞Mg~(2+)-ATP酶、G-6-P酶活性的影响。[方法]将实验大鼠随机分为4组:空白对照组、鳖甲煎丸煎剂组、顺铂组、鳖甲煎丸联合顺铂用药组。待人肝癌HEPG2细胞贴壁生长状态良好,加入含药大鼠血清及对照组大鼠血清,培养后取材制作标本。利用Mg~(2+)-ATP酶、G-6-P酶细胞化学染色方法对标本进行染色。运用光镜观察酶反应。[结果]光镜下,鳖甲煎丸煎剂组、顺铂组、鳖甲煎丸联合顺铂用药组Mg~(2+)-ATP酶、G-6-P酶活性减弱,与空白对照组相比较有明显差异,差别有统计学意义(P<0.05)。[结论]鳖甲煎丸含药大鼠血清对人肝癌HEPG2细胞Mg~(2+)-ATP酶、G-6-P酶活性的影响有减弱作用。 [Objective] To investigate the effect of BJJ serum on the activity of Mg ~ (2 +) - ATPase and G-6-P in human hepatoma HEPG2 cells. [Method] The experimental rats were randomly divided into 4 groups: blank control group, Biejiaochang Pill decoction group, Cisplatin group, Biejia decoction combined with Cisplatin group. Hepatocellular carcinoma HEPG2 cells adherent growth well, the drug-containing rat serum and control group were added to the serum after the preparation of specimens drawn. The specimens were stained with Mg ~ (2 +) - ATPase and G-6-P enzyme cytochemical staining. Using light microscopy enzyme reaction. [Result] Under light microscope, the activities of Mg (2 +) - ATPase and G-6-P in BJJ decoction group, Cisplatin group, Biejiajian Pills combined with Cisplatin group were weaker than those in blank control group There were significant differences between the two groups (P <0.05). [Conclusion] The effect of BJJ serum on the activity of Mg ~ (2 +) - ATPase and G-6-P in human hepatocellular carcinoma HEPG2 cells has a weakened effect.