论文部分内容阅读
目的 通过优化前处理方法和气相色谱-串联质谱(GC-MS/MS)仪器参数,建立QuEChERS结合GC-MS/MS法同时测定4种水果中4-氯苯氧乙酸、2,4-二氯苯氧乙酸、萘乙酸、吲哚乙酸、吲哚丁酸5种植物生长调节剂残留量的分析方法.方法 苹果、橘子、香蕉、葡萄样品经二氯甲烷提取,加入三氟化硼甲醇溶液后选用乙二胺-N-丙基硅烷(PSA)+C18进行净化,加温衍生后,采用GC-MS/MS法对5种植物生长调节剂进行定性、定量分析.结果 采用改进的QuEChERS前处理方法,5种植物生长调节剂在10.00～1 000 μg/L浓度之间线性良好(r≥0.999),在10.0 ～200 μg/kg添加水平下,方法的回收率在63.4％～107％之间,相对标准偏差在0.480％ ～ 14.8％之间.结论 该方法快速、准确,结合GC-MS/MS法能满足水果中5种植物生长调节剂残留的检测分析要求.“,”Objective To establish a method for the determination of 4-chlorophenoxyacetic acid,2,4-dichlorophenoxyacetic acid,naphthylacetic acid,indole acetic acid and indolybutyric acid in fruits by QuEChERS with gas chromatography-tandem mass spectrometry (GC-MS/MS).Methods Five plant growth regulators in fruits were extracted by methylene chloride,purified by QuEChERS,and were identified and quantified by GC-MS/MS after derivatization from the purified samples.Results The linear correlation coefficients of each linear regression equation for the five plant growth regulators were over 0.999 and the relative standard deviations (n =6) were between 0.480％ and 14.8％.The recoveries of the method were between 63.4％ and 107％ at the spiked levels of 10.0-200 μg/kg.Conclusion The method was rapid and accurate,and could meet the requirement for the detection of the five kinds of plant growth regulators in fruits.