论文部分内容阅读
目的:探讨海洛因静脉自身给药大鼠中脑腹侧被盖区(VTA),伏隔核(NAc),杏仁核(Amy),前额叶皮层(PFC),黑质(SN),尾壳核(CPu)等脑区内生化功能的改变以及电针的干预作用.方法:建立大鼠海洛因静脉自身给药模型30只,随机分为模型组(n=6),自然戒断组(n=12),电针组(n=12),同时设生理盐水对照组(n=6).自然戒断组中6只大鼠停药戒断7d,另6只大鼠停药戒断14d;电针组停药戒断的同时,每天给予电针治疗,其中6只大鼠治疗7天,另6只大鼠治疗14d;生理盐水组采用生理盐水代替海洛因进行给药训练,余同模型组.采用免疫组化法测定VTA,NAc,SN CPu,Amy,PFC等脑区的神经胶质纤维酸性蛋白(GFAP)及酪氨酸羟化酶(TH)含量.结果:VTA内TH,GFAP含量发生明显上调,NAc内TH, GFAP含量相对下调;而SN, CPu,Amy,PFC内TH, GFAP含量没有改变;电针治疗可加速VTA脑内上调的TH, GFAP含量向正常水平恢复,一定程度逆转受损伤的DA神经元.结论:大鼠海洛因依赖时,VTA-NAc DA通路发生了一定程度的生化功能适应性改变;电针对阿片类药物成瘾后VTA内DA神经元损伤修复和功能恢复具有一定的促进作用.,Objective: This present study is to investigate the biochemical adaptations in the related brain regions of the mesolimbic dopamine system, such as the ventral teental area (VTA), nucleus accumbens (Nac), amygdale (Amy), prefrontal cortex (PFC) , substantia nigra (SN) and caudateputamen (Cpu) in response to heroin self-administration in rats and observe the effect of electroacupuncture on them. Methods: 2hirty rats were trained by nose-poking response to establish stable intravenous heroin self-administration within 14 days, and then divided randomly into model group (group B) including 6 rats, withdrawal group which were withdrawn from heroin for 1 week (group C, n = 6) and for 2 weeks (group D, n= 6), during which time they only lived in their individual home cages,and electro-acupuncture group which were also withdrawn from heroin for 1 week (group E, n= 6) and for 2 weeks (group F, n= 6), during which time they were given electro-acupuncture treatment for 20 min daily and then reted to their individual home cages; in the meantime, another 6 rats were trained by nose-poking response with saline for 14 days as control (group A); Then the levels of tyrosine hydroxylase (TH) and glial fibrillary acidic protein (GFAP) in VTA, Nac, Amy, PFC, SN, Cpu were detected with immunohistochemistry method. Results: The leveks of TH and GFAP in VTA of the heroin self-administrating rats were obviously increased, and the levels of TH and GFAP in Nac were also decreased, and these changes were not found in SN, Cpu, Amy and PFC; Electro-acupuncture could promote the up-regulation of TH and GFAP in VTA and down-regulation of TH and GFAP in Nac to ret to the normal level. Conclusions: The chronic heroin self-administration produced some biochemical adaptations in the rdated brain regions of the mesolimbic dopamine system and electroacupuncture could promote the repair of the injured DA neurons in VTA of heroin addicted rats and their functional recovery.