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免疫球蛋白Ig是由两条重链及两条轻链组成的糖蛋白,分别由定位于不同染色体上的3个不连锁的Igc,Igλ,IgH基因所编码.目前的免疫学理论认为,在正常的机体内,Ig基因只有在B细胞来源的细胞中进行选择性重排及表达,是B淋巴细胞的特有产物.然而,我们曾用免疫学方法检测表明癌细胞可能表达免疫球蛋白.近期设计了23条PCR引物,分别位于Ig重链前导,肽区,FRIJ链区,恒定区,并用RTPCB的方法对4个来源于人的恶性肿瘤细胞系HT-29(大肠癌),MCF-7(乳腺癌),HeLaMR(宫颈癌),PC3M(前列腺癌)及来源于健康人外周血的单个核细胞的阳性对照进行了检测.
Immunoglobulin Ig is a glycoprotein composed of two heavy chains and two light chains, encoded by three non-linked Igc, Igλ, IgH genes located on different chromosomes. Current immunological theory holds that In the normal body, the Ig gene is selectively rearranged and expressed only in cells derived from B cells and is a unique product of B lymphocytes. However, we have used immunological assays to show that cancer cells may express immunoglobulins. Twenty-three PCR primers were designed, which are located in the Ig heavy chain leader, peptide region, FRIJ chain region and constant region, and used RTPCB method for four human-derived malignant tumor cell lines HT-29 (colorectal cancer), MCF-7. (Breast cancer), HeLa MR (cervical cancer), PC3M (prostate cancer) and positive controls derived from peripheral blood mononuclear cells in healthy individuals were tested.