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血栓素与前列环素是影响血管舒缩,作用相反的一对血管活性物质。为了解血栓素及前列环素在急性肾衰中的作用,我们研究了急性肾衰大鼠肾组织及血浆血栓素及前列环素代谢的改变,观察了樟柳碱,东莨菪碱及山莨菪碱对这些改变的影响。 一、材料与方法 选用中国军事医学科学院实验动物中心提供Wistar雄性大鼠,体重180～250克。采用我们以往报道的直接从左肾动脉注射油酸的方法建立大鼠急性肾衰模型[中华实验外科杂志,1990,7:180]。按文献报道的放射免疫分析法测定急性肾衰后24及48小时及应用樟柳碱、东莨菪碱及山莨菪碱治疗后肾组织及血浆TXB_2和6-keto-PGF_(1a)(6kF)的含量。
Thromboxans and prostacyclin are a pair of vasoactive substances that affect vasomotor and contractions. In order to understand the role of thromboxane and prostacyclin in acute renal failure, we investigated the changes of thromboxane and prostacyclin metabolism in renal tissues and plasma in acute renal failure rats. We observed the effects of anisodine, scopolamine and anisodamine The impact of these changes. First, the selection of materials and methods Experimental Animal Center of Chinese Academy of Military Medical Sciences Wistar male rats, weighing 180 to 250 grams. Acute renal failure model was established by injecting oleic acid directly from the left renal artery in our previous reports [Chinese Journal of Experimental Surgery, 1990, 7: 180]. The levels of TXB2 and 6-keto-PGF1a (6kF) in renal tissue and plasma were measured by radioimmunoassay in the literature at 24 and 48 hours after acute renal failure and after treatment with anisodine, scopolamine and anisodamine.