有机化学反应中活性中间体的性质各异,对反应中间体的表征和研究对于阐明和确证反应机理、优化反应条件、筛选催化剂和发现新类型反应至关重要.近年来,质谱技术和在线微量采样技术的发展,极大程度地拓展了质谱学在该领域的应用.本文总结了近年来本领域发展的新策略、技术和方法,以及有机质谱技术在反应中间体研究中的应用. The properties of the active intermediates in organic reactions are different, and the characterization and research on the intermediates of the reaction are very important for elucidating and confirming the reaction mechanism, optimizing the reaction conditions, screening the catalysts and discovering new types of reactions.In recent years, mass spectrometry The development of sampling technology has greatly expanded the application of mass spectrometry in this field.This paper summarizes the recent development of new strategies, techniques and methods in the art, as well as the application of organic mass spectrometry in the research of reaction intermediates.