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目的：探讨人膀胱癌多药耐药细胞凋亡诱导途径。方法：应用免疫细胞化学、原位ＤＮＡ末端转移酶标记法、相差显微镜及电镜技术，观察ＢＩＵ－８７／ＡＤＭ细胞表面Ｆａｓ抗原的表达和抗ＡＰＯ－１单抗对该细胞存活的影响及其特征。结果： Ｆａｓ抗原在ＢＩＵ－８７／ＡＤＭ中有高表达，经抗ＡＰＯ－１单抗作用后，ＢＩＵ－８７／ＡＤＭ细胞存活率明显降低，光镜及电镜下观察到凋亡细胞的形态学改变，原位ＤＮＡ末端转移酶标记阳性。结论：抗ＡＰＯ－１单抗能与肿瘤细胞表面的Ｆａｓ抗原结合，触发人膀胱癌多药耐药细胞凋亡。
Objective: To investigate the induction of apoptosis in human bladder cancer multidrug-resistant cells. Methods: The expression of Fas antigen on BIU-87 / ADM cells and the effect of anti-APO-1 McAb on the cell survival and its characteristics were observed by immunocytochemistry, in situ DNA end-transferase labeling, phase contrast microscopy and electron microscopy . RESULTS: Fas antigen was highly expressed in BIU-87 / ADM cells. The viability of BIU-87 / ADM cells was significantly decreased after anti-APO-1 mAb treatment. Morphological changes of apoptotic cells were observed under light microscope and electron microscope , In situ DNA transferase labeling positive. CONCLUSION: Anti-APO-1 mAb can bind to Fas antigen on the surface of tumor cells and trigger the apoptosis of multidrug-resistant human bladder cancer cells.