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目的：为探讨细胞毒素相关蛋白（CagA）与幽门螺杆菌(Hp)相关胃十二指肠疾病的关系及其可能的机制。方法：应用间接ELISA法定性检测170例胃镜受检查者CagAHpIgG抗体，用双抗体夹心ELISA法检测其中76例患者胃粘膜匀浆上清液中的白细胞介素8（IL－8）含量。结果：170例受检者中，Hp感染者106例，其中CagA阳性69例，阳性率为6509% ，Hp感染的DU、GU、GCa患者CagA阳性率明显高于Hp感染的CG患者（P<0.05）, DU、GU、GCa组间无明显差异（P>0．05）;活动性胃炎CagA阳性率与非活动性胃炎相比较无显著性差别（P＞0，05）；血清CagA阳性的Hp感染者胃粘膜IL8含量显著高于CagA阴性者（P<0.05），后者又显著高于非Hp感染者（P<0．005）；活动性胃炎胃粘膜IL－8含量明显高于非活动性胃炎（P<0.05）。结论：CagA与Hp相关的严重胃十二指肠疾病密切相关，其机制可能与CagA介导胃粘膜IL8的产生，后者参与Hp相关性活动性胃炎的病理过程有关。
Objective: To investigate the relationship between CagA and Helicobacter pylori (Hp) related gastroduodenal diseases and its possible mechanism. Methods: Indirect ELISA was used to detect 170 cases of CagAHpIgG antibody in gastroscopy. The content of interleukin 8 (IL-8) in gastric mucosa supernatant of 76 patients was detected by double antibody sandwich ELISA. Results: Of the 170 subjects, 106 were infected with Hp, of which 69 were positive for CagA and the positive rate was 6509%. The positive rates of CagA in Hp infected DU, GU and GCa patients were significantly higher than those in Hp infected CG patients (P < 0.05). There was no significant difference between DU, GU and GCa groups (P> 0.05). The positive rate of CagA in active gastritis was not significantly different from that in inactive gastritis (P> 0.05) The content of IL8 in gastric mucosa of patients with Hp infection was significantly higher than that of CagA negative patients (P <0.05), while the latter was significantly higher than that of non-Hp infected patients (P <0.005). The content of IL-8 in gastric mucosa of patients with active gastritis was significantly higher than that of non- Active gastritis (P <0.05). CONCLUSION: CagA is closely related to Hp-related severe gastroduodenal disease, and its mechanism may be related to CagA-mediated gastric mucosal IL8 production, which is involved in the pathological process of Hp-related active gastritis.