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目的探计AgNOR染色对胸腹水涂片中良、恶性细胞诊断和鉴别诈断的意义。方法应用银染方法对20例癌性胸腹水涂片和10例良性胸腹水涂片进行了AgNOR检测和观察。结果癌细胞核内的AgNOR颗粒平均数为7.2±1.38,明显高于增生间皮细咆核内的AgNOR平均数1.36±0.21,两组间差异有高度显著性(P<0.001);在形态上癌细胞颗粒大小不一,形态不规,畸形明显,以聚集型和弥散型为主,增生间皮细胞的颗粒形态规则。大小一致,以单一型为主。结论应用AgNOR颗粒计数结合颗粒形态观察分型综合分析对胸腹水中良、恶性细胞的鉴别诊断具有一定的实用价值。
Objective To explore the significance of AgNOR staining in the diagnosis and differential diagnosis of benign and malignant cells in thoracic and abdominal smears. Methods AgNOR was used to detect and observe 20 cases of cancerous hydrothorax and ascites smears and 10 cases of benign pleural effusions. Results The average number of AgNOR particles in the cancer cell nucleus was 7.2±1.38, which was significantly higher than that of the AgNOR in the hyperplastic mesothelial nucleus 1.36±0.21. The difference between the two groups was highly significant (P<0.001); The cell granules are of different sizes, irregular morphology, and obvious deformities. The main types of aggregated and diffused granules are the granular shape of the proliferating mesothelial cells. The same size, mainly in a single type. Conclusion AgNOR particle counting combined with particle morphological observation and comprehensive analysis has certain practical value in the differential diagnosis of benign and malignant cells in thoracic and abdominal water.