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【目的】为了解油樟产芽孢内生细菌的多样性。【方法】采用改良的牛肉膏琼脂培养基分离、去除冗余及芽孢染色,测定所得产芽孢内生细菌的16S rRNA基因,进行系统发育分析。【结果】40株产芽孢内生细菌数量占分离得到的内生细菌总数的38.1%,其中根、茎、叶中分别分离得到24株、7株和9株。16S rRNA基因序列系统发育分析结果表明,35株菌可能分属于Bacillus、Lysinibacillus、Paenibacillus属的16个种,还有5株菌的序列与数据库中典型菌株序列相似性低于97%,代表着潜在新类群的存在。【结论】从油樟3个部位分离出的产芽孢内生细菌存在明显的系统发育多样性,而且3个部位分离出的产芽孢内生细菌区系既呈现出一定程度的细菌区系相似性,又表现出器官细菌区系的特异性。
【Objective】 To understand the diversity of endophytic bacteria in camphor oil camphor. 【Method】 The modified beef extract agar medium was used to separate and eliminate the redundant and spore staining. The 16S rRNA gene of endophytic bacteria was determined and phylogenetic analysis was performed. 【Result】 The results showed that the number of endophytic bacteria in 40 strains accounted for 38.1% of the total number of endophytic bacteria isolated, of which 24 strains, 7 strains and 9 strains were isolated from roots, stems and leaves. Phylogenetic analysis of 16S rRNA gene sequences showed that 35 strains could belong to 16 species of Bacillus, Lysinibacillus and Paenibacillus, and the similarity of other 5 strains to the typical strain in the database was less than 97%, indicating potential The existence of new taxa. 【Conclusion】 There are obvious phylogenetic diversity of endophytic bacteria isolated from three parts of Campylobacter camphora and three isolates of endophytic bacteria isolated from the spores show some degree of bacterial flora similarity , But also shows the specificity of organ bacterial flora.