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目的同时测定赶黄草中活性成分β-谷甾醇、槲皮素及没食子酸的含量。方法粉碎后的赶黄草采用乙醇溶液浸提、过滤,滤液浓缩后用甲醇溶解获得供试品溶液。采用高效液相色谱法,SunFireC18色谱柱,以乙腈-0.1%磷酸溶液为流动相梯度洗脱,流速为1.0 ml/min,检测波长为254 nm。利用标准曲线法定量。结果β-谷甾醇在1.40~3.50μg、槲皮素在0.42-4.20μg、没食子酸在0.32~6.40μg范围内线性关系良好,β-谷甾醇的回收率为99.08%,RSD为1.50%;槲皮素的回收率为99.06%,RSD为0.98%;没食子酸的回收率为98.58%,RSD为1.04%。β-谷甾醇、槲皮素、没食子酸的检出限分别为2.8、3.4、2.4 ng。结论该方法简便、分离效果好、结果准确,适于赶黄草中β-谷甾醇、槲皮素及没食子酸的含量测定。
Objective To determine the content of β-sitosterol, quercetin and gallic acid in active ingredient of Phellinus igniarius simultaneously. Method crushed catch yellow grass with ethanol solution leaching, filtration, the filtrate was concentrated with methanol to obtain the test solution. Using high performance liquid chromatography, SunFire C18 column was eluted with acetonitrile-0.1% phosphoric acid as the mobile phase with a flow rate of 1.0 ml / min and a detection wavelength of 254 nm. Using standard curve method. Results β-sitosterol ranged from 1.40 to 3.50 μg, quercetin ranged from 0.42 to 4.20 μg, and gallic acid ranged from 0.32 to 6.40 μg. The recovery rate of β-sitosterol was 99.08% and the RSD was 1.50% The recovery rate of verapamil was 99.06% with RSD of 0.98%. The recovery of gallic acid was 98.58% with RSD of 1.04%. The detection limits of β-sitosterol, quercetin and gallic acid were 2.8, 3.4 and 2.4 ng, respectively. Conclusion The method is simple, the separation effect is good and the result is accurate. It is suitable for the content determination of β-sitosterol, quercetin and gallic acid in Phellinus igniarius.