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Superoxide dismutase(SOD) is a crucial antioxidant enzyme playing the first defense line in antioxidant pathways against reactive oxygen species in various organisms including marine invertebrates. There exist mainly two specific forms, Cu/Zn-SOD(SOD1) and Mn-SOD(SOD2), in eukaryotes. SODs are known to be concurrently modulated by a variety of environmental stressors. By using central composite experimental design and response surface method, the joint effects of water temperature(18–34°C) and copper ion concentration(0.1–1.5 mg/L) on the total SOD activity in the digestive gland of Crassostrea ariakensis were studied. The results showed that the linear effect of temperature was highly significant(P<0.01), the quadratic effect of temperature was significant(P<0.05); the linear effect of copper ion concentration was not significant(P>0.05), while the quadratic effect of copper ion concentration was highly significant(P<0.01); the interactive effect of temperature and copper ion concentration was not significant(P>0.05); the effect of temperature was greater than that of copper ion concentration. The model equation of digestive gland SOD enzyme activity towards the two factors of interest was established, with R2 and predictive R2 as high as 0.961 6 and 0.820 7, respectively, suggesting that the goodness-offit to experimental data be very satisfactory, and could be applied to prediction of digestive gland SOD activity in C. ariakensis under the conditions of the experiment. Our results would be conducive to addressing the health of aquatic animals and/or to detecting environmental problems by taking SOD as a potential bioindicator.
Superoxide dismutase (SOD) is a crucial antioxidant enzyme playing the first defense line in antioxidant pathways against reactive oxygen species in various organisms including marine invertebrates. There exist mainly two specific forms of Cu / Zn-SOD (SOD1) and Mn-SOD By using central composite experimental design and response surface method, the joint effects of water temperature (18-34 ° C) and copper ion concentration (0.1- 1.5 mg / L) on the total SOD activity in the digestive gland of Crassostrea ariakensis were studied. The results showed that the linear effect of temperature was highly significant (P <0.01), the quadratic effect of temperature was significant (P <0.05) ; the linear effect of copper ion concentration was not significant (P> 0.05), while the quadratic effect of copper ion concentration was highly significant (P <0.01); the interactive effect of temperature and copper ion con The effect of temperature was greater than that of copper ion concentration. The model equation of digestive gland SOD enzyme activity towards the two factors of interest was established, with R2 and predictive R2 as high as 0.961 6 and 0.820 7, respectively, suggesting that the good-off to the experimental data be very satisfactory, and could be applied to prediction of digestive gland SOD activity in C. ariakensis under the conditions of the experiment. Our results would be conducive to addressing the health of aquatic animals and / or to detect environmental problems by taking SOD as a potential bioindicator.