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The expressions of electron density fluctuation produced by dust particles in a dusty plasma are derived. It is shown that the fluctuation comes from three different sources. The first source is the thermal motion of the dust particles, which is of a fluctuation power spectrum proportional to ndZ2. The second source is the charge change of the dust particles, which is of a power spectrum proportional to n2dZ3. The third source is the eigenmode oscillation of the dust particles, which is of fluctuation power spectrum proportional to √ nd. The powerful electron density fluctuation exists in a dusty plasma due to the fact that the dust particles are highly charged.The results may explain the strong electromagnetic scattering by a space dust layer in the Earths mesosphere.