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目的 研究血小板衍生生长因子（ＰＤＧＦ）及受体（ＰＤＧＦＲ）在慢性肝炎纤维化和肝硬化患者肝组织中的表达、分布和意义。方法 用免疫组织化学方法检测了２１例慢性肝炎、４２例肝硬化患者肝组织中ＰＤＧＦ—Ａ、ＰＤＧＦ－Ｂ、ＰＤＧＦＲ－α、ＰＤＧＦＲ－β及α－平滑肌肌动蛋白（α－ＳＭＡ）的表达、分布状况、定量和相关性分析。结果 慢性肝炎和肝硬化组织ＰＤＧＦ及其受体和α－ＳＭＡ的表达主要分布于汇管区、纤维间隔和炎细胞浸润聚集区，尤其见带分支突起的梭形细胞（活化的ＨＳＣ）有大量表达。ＰＤＧＦ－Ｂ和ＰＤＧＦＲ－β表达分别强于ＰＤＧＦ－Ａ和ＰＤＧＦＲ－α，两组间差异有显著性（Ｐ＜０．０５～０．０１）。α－ＳＭＡ与 ＰＤＧＦ－Ａ ＰＤＧＦ－Ｂ及ＰＤＧＦＲ－α、ＰＤＧＦＲ－β的表达、分布基本一致，定量分析呈正相关，相关系数分别为 ０．６０６（Ｐ＜０．００１）、０．７７２（Ｐ＜０．００１）、０．６８４（Ｐ＜０．００１）、０．８２５（Ｐ＜０．００１）。结论 ＰＤＧＦ及其受体在肝纤维化发生发展中起重要作用，是通过活化 ＨＳＣ发挥效应，研究抑制ＰＤＧＦ及其受体的产生和作用是防治肝纤维化的新途径。
Objective To study the expression, distribution and significance of platelet-derived growth factor (PDGFR) and receptor (PDGFR) in liver tissues of chronic hepatitis with fibrosis and cirrhosis. Methods The expression of PDGF-A, PDGFR-α, PDGFR-β and α-smooth muscle actin (α-SMA) in 21 patients with chronic hepatitis and 42 patients with liver cirrhosis was detected by immunohistochemistry , Distribution, quantitative and correlation analysis. Results The expression of PDGF, its receptor and α-SMA in chronic hepatitis and cirrhosis mainly distributed in portal area, fibrocyte and inflammatory cell infiltration area, especially in spindle-shaped cells with activated branches (activated HSC) . The expressions of PDGF-B and PDGFR-β were stronger than those of PDGF-A and PDGFR-α respectively (P <0.05-0.01). The expression and distribution of α-SMA and PDGF-A PDGF-B, PDGFR-α and PDGFR-β were basically the same, the correlation was 0.606 (P <0.001), 0.772 <0.001), 0.684 (P <0.001), 0.825 (P <0.001). Conclusion PDGF and its receptors play an important role in the development of hepatic fibrosis and exert its effect through activation of HSC. It is a new way to prevent and treat hepatic fibrosis by inhibiting the production and function of PDGF and its receptors.