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将从柽柳cDNA文库中分离的eIF1A基因采用农杆菌介导法进行山新杨的遗传转化。经PCR及Northern检测,证明了该基因已经整合到山新杨基因组中,并且能够在转录水平上得到表达。盐胁迫处理表明:盐胁迫处理7 d后,转基因山新杨净光合速率(Pn)、气孔导度平均值(Gs)分别为对照的1.33、1.27倍。光化学猝灭系数(qp)、PSⅡ电子传递量子产率(ΦPSⅡ)的平均值是对照株系的1.49、1.10倍。胁迫处理15 d后,转基因株系与非转基因株系相比,所受的盐害较小,而高生长均高于对照株系,其平均值是对照的1.26倍。这些指标的变化均证实eIF1A基因的表达能够提高转基因山新杨抵抗盐胁迫的能力。
Agrobacterium-mediated transformation of eIF1A gene isolated from Tamarix elongatum cDNA library was used to transform S. yunnanensis. The results of PCR and Northern blot showed that the gene has been integrated into the genome of Populus simonii and expressed at the transcriptional level. Salt stress treatment showed that the net photosynthetic rate (Pn) and stomatal conductance (Gs) of P. xylostella were 1.33 and 1.27 times higher than that of the control respectively after 7 days of salt stress. The average values of photochemical quenching (qp) and PSⅡ electron transfer quantum yield (ΦPSⅡ) were 1.49 and 1.10 times that of the control. Compared with the non-transgenic lines, the transgenic lines were less salt tolerant and higher than the control lines after 15 days of stress treatment. The average value was 1.26 times of the control. Changes in these indicators confirm that the expression of eIF1A gene can increase the resistance of transgenic poplar to salt stress.