论文部分内容阅读
目的探讨宫颈癌患者中脆性组氨酸三联体基因(fragile histidine triad,FHIT)CpG岛甲基化状态及其在宫颈癌诊断中价值。方法采用甲基化特异性PCR技术检测45例宫颈癌组织及血浆中、10例正常宫颈组织中FHIT基因CpG岛甲基化状态。结果 FHIT基因在宫颈癌组织及血浆中CpG岛甲基化率分别为55.6%和35.6%,正常宫颈组织中未检测出FHIT基因CpG岛甲基化,宫颈癌组织及血浆中CpG岛甲基化改变与正常对照比较差异有统计学意义(P<0.05);癌组织中FHIT基因甲基化发生率与年龄、病理分级、临床分期间差异无统计学意义(P>0.05)。结论 FHIT基因CpG岛甲基化检测在宫颈癌早期诊断中有一定价值。
Objective To investigate the methylation status of fragile histidine triad (FHIT) CpG island in patients with cervical cancer and its value in the diagnosis of cervical cancer. Methods The methylation status of FHIT gene CpG island in 45 cases of cervical cancer and 10 cases of normal cervical tissue was detected by methylation-specific PCR. Results The methylation rates of CpG island of FHIT gene in cervical cancer tissues and plasma were 55.6% and 35.6% respectively. No methylation of CpG island of FHIT gene, methylation of CpG island in cervical cancer tissues and plasma were detected in normal cervical tissues (P <0.05). The incidence of methylation of FHIT gene in cancer tissues was not significantly different from that in age, pathological grade and clinical stage (P> 0.05). Conclusion Methylation detection of CpG island of FHIT gene has some value in the early diagnosis of cervical cancer.