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为探讨喉癌细胞本身的生物学指标与临床特征的联系,采用流式细胞分析技术检测了喉癌细胞的DNA含量(即异倍体率,RH)、s-期细胞(SPC)百分比;分别采用免疫组化ABC法和银染图像分析技术检测了增殖细胞核抗原(PCNA)标记指数及核仁组成区(AgNOR)的面积。结果表明颈淋巴结转移组(11例)RH、SPC、PCNA、AgNOR分别为76%、22.43±5.12%、88.51±14.21%和4.854±2.357μm~2,显著高于非转移组(46例)的68%、17.74±5.41%、81.43±14.03%和4.103±3.552μm~2(P<0.05);低分化鳞癌组(20例)RH、SPC、PC-NA、AgNOR分别为71%、17.43±5.12%、77.49±15.18%和5.018±3.142μm~2,显著高于高分化组(19例)的65%、12.74±5.41%、68.32±11.12%和4.517±3.215μm~2(P<0.05);喉癌Ⅳ期(18例)RH 75%显著高于喉癌工期(9例)的68%(P<0.05)。结论:RH、SPC、PCNA、AgNOR是反映喉癌细胞生物学行为的较好指标,对了解病情、预测颈淋巴结转移有一定帮助。
To investigate the relationship between biological indicators and clinical features of laryngeal cancer cells, the DNA content (ie aneuploidy rate, RH) and the percentage of s-phase cells (SPC) of laryngeal cancer cells were detected by flow cytometry Immunohistochemical ABC method and silver staining were used to detect the proliferating cell nuclear antigen (PCNA) labeling index and the area of nucleolar organizer area (AgNOR). The results showed that the RH, SPC, PCNA and AgNOR were 76%, 22.43 ± 5.12%, 88.51 ± 14.21% and 4.854 ± 2.357μm2 in cervical lymph node metastasis group (11 cases), which were significantly higher than those in non-metastatic group (46 cases) 68%, 17.74 ± 5.41%, 81.43 ± 14.03% and 4.103 ± 3.552μm 2 respectively (P <0.05). The RH, SPC, PCNA and AgNOR in poorly differentiated squamous cell carcinoma group were 71%, 17.43 ± (P <0.05), which were significantly higher than that of the well-differentiated group (5.12%, 77.49 ± 15.18% and 5.018 ± 3.142μm 2, respectively), which were significantly higher than 65%, 12.74 ± 5.41%, 68.32 ± 11.12% and 4.517 ± 3.215μm 2, ; Laryngeal stage Ⅳ (18 cases) RH 75% was significantly higher than the laryngeal cancer stage (9 cases) 68% (P <0.05). CONCLUSIONS: RH, SPC, PCNA and AgNOR are good indicators of biological behavior of laryngeal cancer cells. They are helpful to understand the condition and predict cervical lymph node metastasis.