论文部分内容阅读
目的:检测白细胞中血管紧张素原(AGT)MRNA的量.方法:提取总RNA,进行NORTHERN印迹杂交;应用RT－PCR技术联合印迹杂交和同位素掺入法对微量MRNA检测.结果:NORTHERN印迹杂交显示肝细胞血管紧张素原MRNA有明显的杂交带,而白细胞无杂交信号.将RT－PCR技术同印迹杂交或同位素掺入法联合应用能提高血管紧张素原微量MRNA检测灵敏度.结论:白细胞的AGTMRNA量低于肝细胞;RT－PCR联合印迹杂交或同位素掺入法是微量MRNA定量分析的有效方法.“,”Objective: To detect the quantity of angiotensinogen mRNA in leukocytes. Methods:Total RNA was extracted and used for northern blotting. RT PCR with blotting and isotope labeling were used to detect minor amount of mRNA. Results: Northern blotting demonstrated that the angiotensinogen mRNA in hepatic cells presented clear band and no signal in leukocytes. The angiotensinogen mRNA in leukocytes can be detected by RT PCR with blotting and isotope labeling. Conclusion: The amount of angiotensinogen mRNA in l...