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目的观察碱性成纤维生长因子(bFGF)基因修饰的间充质干细胞(MSCs-bFGF)对大鼠脑缺血的保护作用。方法将间充质干细胞(MSCs)或 MSCs-bFGF 通过静脉移植至实验性脑缺血大鼠体内,比较两者对大鼠脑缺血后神经功能及脑梗死体积的影响,并应用双重荧光标记方法观察移植细胞在脑内的存活和分化情况。结果静脉移植 MSCs 和 MSCs-bFGF 均能改善脑缺血后大鼠的神经功能、减少脑梗死体积,且 MSCs-bFGF 的作用明显优于 MSCs,MSCs-bFGF 移植7 d 后大鼠脑内BrdU 阳性细胞数、BrdU-NeuN 双标阳性细胞数分别为127.40±7.43、11.20±3.09,明显多于 MSCs 治疗组大鼠,而 BrdU—GFAP 双标阳性细胞数两组间差异无统计学意义。结论 MSCs 移植对脑缺血具有保护作用,bFGF 基因修饰可提高 MSCs 移植对大鼠脑缺血后损伤的保护和神经功能恢复作用。
Objective To observe the protective effect of basic fibroblast growth factor (bFGF) gene-modified mesenchymal stem cells (MSCs-bFGF) on cerebral ischemia in rats. Methods Mesenchymal stem cells (MSCs) or MSCs-bFGF were transplanted into experimental cerebral ischemia rats by intravenous injection. The effects of both of them on neural function and cerebral infarction volume after cerebral ischemia in rats were compared. Double fluorescent markers Methods The survival and differentiation of transplanted cells in the brain were observed. Results Both MSCs and MSCs-bFGF could ameliorate the neurological function and reduce the volume of cerebral infarction in MSCs-bFGF, and the effect of MSCs-bFGF was better than that of MSCs. After 7 days MSCs-bFGF transplantation, the BrdU positive The number of BrdU-NeuN positive double-labeled cells was 127.40 ± 7.43,11.20 ± 3.09, respectively, which was significantly higher than that of MSCs-treated group. There was no significant difference between the two groups in BrdU-GFAP double positive cells. Conclusion MSCs transplantation has a protective effect on cerebral ischemia. BFGF gene modification can improve the protection of MSCs transplantation and the recovery of neurological function after cerebral ischemia in rats.