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目的探讨黑木耳多糖对小鼠营养性肥胖干预作用及可能机制。方法 40只小鼠随机分为对照组、高脂组、黑木耳多糖低、中、高剂量组,高脂膳食同时灌胃给予黑木耳多糖,连续4周;测定各组小鼠体重、Lee’s指数、脂肪指数、血清中总胆固醇(TC)、总甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、瘦素及激素敏感性脂肪酶(HSL)含量。结果与对照组比较,高脂组小鼠Lee’s指数、脂肪指数[分别为(341.53±4.39)、(3.59±0.83)%]明显升高,血清中TC、TG、瘦素、HSL含量[分别为(5.06±0.10)、(2.12±0.20)mmol/L、(4.00±0.25)、(8.16±0.91)ng/m L]明显升高;与高脂组比较,黑木耳多糖高剂量组(100 mg/kg)小鼠Lee’s指数、脂肪指数[(328.48±4.16)、(2.37±0.14)%]下降,血清中TC、TG、瘦素含量[(3.50±0.21)、(1.71±0.17)mmol/L、(3.72±0.55)ng/m L]下降,黑木耳多糖中剂量组(50 mg/kg)小鼠血清中HSL含量[(7.79±0.25)ng/m L]下降。结论黑木耳多糖对小鼠高脂膳食所致营养性肥胖具有干预作用,其机制可能与降低小鼠血清中瘦素及激素敏感性脂肪酶水平有关。
Objective To investigate the effect of black fungus polysaccharide on nutritional obesity in mice and its possible mechanism. Methods Forty mice were randomly divided into control group, high fat group, black fungus polysaccharide low, medium and high dose group, high fat diet while gavage black fungus polysaccharide continuously for 4 weeks; body weight, Lee’s index , Fat index, total cholesterol (TC), total triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), leptin and hormone sensitive lipase (HSL) Results Compared with the control group, the Lee’s index and fat index [(341.53 ± 4.39) and (3.59 ± 0.83)%] in hyperlipidemic mice were significantly increased, and the content of TC, TG, leptin and HSL in serum were (5.06 ± 0.10), (2.12 ± 0.20) mmol / L, (4.00 ± 0.25) and (8.16 ± 0.91) ng / m L, respectively. Compared with the hyperlipemia group, (P <0.05). The Lee’s index and fat index [(328.48 ± 4.16) and (2.37 ± 0.14)%] , (3.72 ± 0.55) ng / m L], while the content of HSL in the serum of the black fungus polysaccharide medium dose group (50 mg / kg) [(7.79 ± 0.25) ng / m L] decreased. Conclusion Auricularia polysaccharides can interfere with the nutritional obesity induced by high-fat diet in mice. The mechanism may be related to the decrease of serum leptin and hormone-sensitive lipase.