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将玻碳电极(GCE)放入含有L-色氨酸(TR)和氧化石墨烯(GO)底液进行循环扫描聚合,得到聚L-色氨酸(PTR)和电化学还原石墨烯(ERGO),从而制备了PTR-ERGO/GCE,电极具有较快的电子传递速率和较好的催化能力。利用循环伏安法(CV)和差分脉冲伏安法(DPV)探究尿酸(UA)和黄嘌呤(Xa)在该电极上的电化学行为。UA和Xa在电极表面的氧化过程均受吸附和扩散共同控制,以扩散为主。在最佳条件下,UA在0.626 V处产生一个氧化峰,Xa在0.994 V处产生一个氧化峰,两峰分开0.368 V,不需分离,即可同时进行测定。采用DPV法同时测定UA和Xa的线性范围分别为5.0×10-8~2.0×10-4mol/L和1.0×10-7~2.0×10-4mol/L,检出限分别为10和30 nmol/L。方法已用于人体尿样中尿酸和黄嘌呤的同时测定。
The glassy carbon electrode (GCE) was placed in a cyclic scanning polymerization of L-tryptophan (TR) and graphene oxide (GO) to obtain poly-L-tryptophan (PTR) and electrochemically reduced graphene (ERGO ) To prepare PTR-ERGO / GCE, the electrode has a faster electron transfer rate and better catalytic ability. The electrochemical behavior of uric acid (UA) and xanthine (Xa) on the electrode was investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The oxidation processes of UA and Xa on the electrode surface are controlled by both adsorption and diffusion, and dominated by diffusion. Under optimal conditions, UA produces an oxidation peak at 0.626 V, Xa produces an oxidation peak at 0.994 V, and the two peaks separate 0.368 V and can be measured simultaneously without separation. The linear range of UA and Xa determined by DPV method was 5.0 × 10-8 ~ 2.0 × 10-4mol / L and 1.0 × 10-7 ~ 2.0 × 10-4mol / L, the detection limits were 10 and 30 nmol / L. The method has been used for the simultaneous determination of uric acid and xanthine in human urine samples.