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目的:优选山药总多糖脂质体的处方工艺并对其进行表征,为该有效部位的开发提供参考。方法:采用逆向蒸发-微孔滤膜挤压法制备山药总多糖脂质体。以卵磷脂质量浓度、卵磷脂与胆固醇比例、药脂比为考察因素,包封率为评价指标,采用L9(34)正交试验设计优选处方。利用苯酚-硫酸法测定山药总多糖含量,高速离心法测定山药总多糖脂质体的包封率。结果:山药总多糖脂质体的最佳处方工艺为卵磷脂-胆固醇(6∶1),卵磷脂质量浓度12 g·L~(-1),药脂比1∶15;包封率和载药量分别为83.29%,3.83%,平均粒径121.6 nm,多分散指数平均值0.136,平均Zeta电位-31.8 m V。结论:优选的处方工艺稳定可行、重复性好。制备的山药总多糖脂质体形态圆整、粒径分布均匀、包封率较高。
OBJECTIVE: To optimize the prescription of yam polysaccharide liposomes and characterize them, providing a reference for the development of this effective fraction. Methods: The yam polysaccharide liposomes were prepared by reverse evaporation - microporous membrane extrusion. Taking lecithin concentration, ratio of lecithin to cholesterol and ratio of drug to lipid as factors and entrapment efficiency as evaluation indexes, the optimal prescription was designed by L9 (34) orthogonal test. The total polysaccharide content of yams was determined by phenol-sulfuric acid method, and the entrapment efficiency of yam polysaccharide liposomes was determined by high-speed centrifugation. Results: The optimum formulation of total lipopolysaccharide of yam was lecithin - cholesterol (6:1), lecithin concentration 12 g · L -1, lipid - to - lipid ratio 1:15, encapsulation efficiency and The doses were 83.29% and 3.83%, respectively. The average particle diameter was 121.6 nm, the average polydispersity index was 0.136 and the average Zeta potential was -31.8 mV. Conclusion: The preferred prescription process is stable and reproducible. The prepared yam polysaccharide liposomes are in the form of round shape, uniform particle size distribution and high entrapment efficiency.