论文部分内容阅读
用环氧氯丙烷（ＥＣ）和（或）ＴｒｉｔｏｎＸ１００分别对戊二醛（ＧＡ）处理的猪主动脉瓣进行化学改性，以单纯ＧＡ处理的猪瓣作对照。经新西兰幼兔皮下埋藏２、４、６、８周后分别应用原子吸收光谱、光镜及电镜观察，对钙质进行定量、定性和形态学研究。发现ＥＣ＋ＴｒｉｔｏｎＸ１００组的钙含量最低、组织形态学示钙化程度最轻，ＥＣ组和ＴｒｉｔｏｎＸ１００组次之，ＧＡ组最重。实验证明：ＥＣ＋ＴｒｉｔｏｎＸ１００联合化学改性ＧＡ处理的猪主动脉瓣能明显提高防钙化的效果。且能保持猪主动脉瓣完整的组织结构并提高猪主动脉瓣组织的稳定性。急性毒性试验证明，经ＥＣ处理和ＥＣ＋ＴｒｉｔｏｎＸ１００联合处理的猪主动脉瓣无毒性反应。
The porcine aortic valves treated with glutaraldehyde (GA) were chemically modified by epichlorohydrin (EC) and / or TritonX-100, respectively. New Zealand young rabbits were subcutaneously buried for 2, 4, 6, 8 and 8 weeks afterwards. Atomic absorption spectroscopy, light microscopy and electron microscopy were used to investigate the quantitative, qualitative and morphological studies of calcium. It was found that the EC + TritonX 100 group had the lowest calcium content, histomorphology showed the lightest calcification, followed by EC group and TritonX100 group and GA group. Experiments show that: EC + TritonX 100 combined with chemically modified GA pig aortic valve can significantly improve the anti-calcification effect. And can maintain the integrity of porcine aortic valve tissue structure and improve the stability of porcine aortic valve tissue. Acute toxicity test proved that EC treatment and EC + TritonX 100 combined treatment of porcine aortic valve non-toxic reactions.