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本试验用人血离体淋巴细胞培养和~3H 同位素示踪技术,探讨了软 X 射线对淋巴细胞 DNA合成能力的影响。试验表明,在22伦至440伦的剂量范围内,软 X 射线照射都可导致淋巴细胞 DNA 合成能力的下降。培养前照射和培养后48小时照射,细胞的 DNA 合成受抑率不一。结果表明,培养前(细胞密度较高)照射,细胞对射线的敏感度要高于培养后照射(~3H-TdR(~3H-胸腺嘧啶核苷)平均相对掺入率分别降为40. 58～70. 04%:82. 73～87. 32%);已启动分裂的、培养48小时后受照射的细胞,比未启动分裂的、培养24小时受照的细胞要敏感一些(~3H-TdR 平均相对掺入率分别为67. 52%:82. 88%)。受软 X 射线照射的淋巴细胞 DNA 合成能力的受抑率与前人用~(60) Co-γ射线和硬 X 射线相比,前者略高于后者,在低剂量范围内,R.B.E.约为1. 1～1. 3。
This experiment using human blood lymphocytes and ~ 3H isotope tracing technology to explore the soft X-ray lymphocyte DNA synthesis ability. Experiments have shown that soft X-ray irradiation results in a decrease in lymphocyte DNA synthesis capacity in the dose range from 22 Lun to 440 Lun. Pre-incubation and irradiation 48 hours after irradiation, the inhibition of DNA synthesis by cells. The results showed that the sensitivity of the cells to radiation before irradiation (higher cell density) was higher than that after irradiation (~ 3H-TdR (~ 3H-thymidine) average relative incorporation decreased to 40. 58 ~ 70.04%: 82.73-87.32%). Cells that had been activated for division after irradiation for 48 hours were more sensitive than those that did not initiate division for 24 hours (~ 3H- TdR average relative incorporation rate was 67. 52%: 82. 88%). The inhibitory rate of DNA synthesis ability of lymphocytes irradiated by soft X-ray was slightly higher than that of ~ (60) Co-γ-ray and hard X-ray irradiation. In the low dose range, RBE was about 1. 1 ~ 1. 3.