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Objective:To determine the occurrence of class A and class Cβ-Iactamase genes and their cooccurrence in Indian Enterobacteriaceae.Methods:52 third generation cephalosporin resistant isolates were phenotypically detected by combination disk method and screened by PCR to identify class A and class C typeβ-lactamase genes.Results:Of the 52 isolates,94.2%(49) were found harboring any of the bla_(?),bla_(CTX-M),bla_(SHV) and bla_(TEM) were present in 82.6%(43/52), 59.6%(31/52),and 42.3%(22/52) isolates,respectively.Of the 49 ESBL positive isolates 57.1% (28/49) showed co-occurrence of bla_(ampC)with bla_(?).On the contrary,the collection from 2009 showed their co-occurrence in 81.4%isolates.Conclusions:The comparative study shows a downward trend for co-existence of bla_(?) with bla_(ampC) from 2009 to 2010.Further large scale studies are needed to address the co-occurrence of class A and class Cβ-lactamases in India and the resistance trend occurring over a period of time.
Objective: To determine the occurrence of class A and class Cβ-Iactamase genes and their cooccurrence in Indian Enterobacteriaceae. Methods: 52 third generation cephalosporin resistant isolates were phenotypically detected by combination disk method and screened by PCR to identify class A and class C type β- Results: Of the 52 isolates, 94.2% (49) were found harboring any of the bla_ (?), bla_ (CTX-M), bla_ (SHV) and bla_ (TEM) were present in 82.6% 52/59), 59.6% (31/52), and 42.3% (22/52) isolates, respectively. Of the 49 ESBL positive isolates, 57.1% (28/49) showed co-occurrence of bla_ (ampC) with bla_ .On the contrary, the collection from 2009 showed their co-occurrence in 81.4% isolates. Conclusions: The comparative study shows a downward trend for co-existence of bla_ (with bla_ (ampC) from 2009 to 2010.Further large scale studies are needed to address the co-occurrence of class A and class Cβ-lactamases in India and the resistance trend occurring over a period of time.