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目的:探讨跑台运动对脑缺血再灌注大鼠海马脑源性神经营养因子(BDNF)、血管内皮生长因子(VEGF)和VEGF受体KDR mRNA表达的影响。方法:使用可逆性阻塞大脑中动脉的方法建立大鼠单侧脑缺血再灌注模型,将符合模型条件的16只大鼠随机分为对照组和运动组,运动方式采取小强度跑台运动,为期1周。另选取8只大鼠作为假手术组。实验后采用实时荧光定量PCR方法测定大鼠海马组织中BDNF、VEGF及KDR mRNA表达。结果:(1)对照组和运动组大鼠缺血侧海马BDNF mRNA表达量显著高于非缺血侧。运动组大鼠缺血侧海马BDNF mRNA表达量显著高于对照组。(2)对照组大鼠海马缺血侧VEGF mRNA表达量显著高于非缺血侧。运动组大鼠两侧海马VEGF mRNA水平分别显著高于对照组。(3)对照组和运动组大鼠缺血侧海马KDR mRNA表达量显著高于非缺血侧。运动组大鼠缺血侧海马KDR mRNA水平显著高于对照组。结论:跑台运动可明显提高缺血再灌注大鼠海马部位BDNF、VEGF及KDR mRNA水平。
Objective: To investigate the effect of treadmill exercise on the expression of brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF) and VEGF receptor KDR mRNA in rats with cerebral ischemia / reperfusion. Methods: A rat model of unilateral cerebral ischemia / reperfusion was established by reversibly occluding the middle cerebral artery (MCAO). Sixteen rats that fit the model conditions were randomly divided into control group and exercise group. The exercise mode adopted low intensity treadmill exercise, For 1 week. Another 8 rats were selected as sham operation group. After the experiment, the expression of BDNF, VEGF and KDR mRNA in hippocampus of rats were detected by real-time fluorescence quantitative PCR. Results: (1) The expression of BDNF mRNA in hippocampus of hippocampus in control group and exercise group was significantly higher than that in nonischemic group. The expression of BDNF mRNA in hippocampus in exercise group was significantly higher than that in control group. (2) The expression of VEGF mRNA in the hippocampus of the control group was significantly higher than that in the non-ischemic side. The level of VEGF mRNA in hippocampus on both sides of exercise group was significantly higher than that of control group. (3) The expression of KDR mRNA in the hippocampus of the control group and exercise group was significantly higher than that of the non-ischemic side. The level of KDR mRNA in hippocampus in exercise group was significantly higher than that in control group. Conclusion: Treadmill exercise can significantly increase the levels of BDNF, VEGF and KDR mRNA in the hippocampus of rats during ischemia / reperfusion.