人用精制Vero细胞狂犬病疫苗为一种安全、有效的新型疫苗 ,我们在研制该种疫苗时首先比较了密度梯度离心法和凝胶过滤柱层析法 ,并发现后者最佳。我们选择了以Sepharose 4FF为介质的凝胶过滤柱层析的工艺 ,并对该方法的上样量、流速等指标进行了选择 ,确定了最佳方法 ,并在研制中使纯化疫苗的杂蛋白去除率达到99 8%以上 ,为大规模生产提供了工艺方法。 Human purified rabies vaccine Vero cells is a safe and effective new vaccine, we first developed the vaccine compared density gradient centrifugation and gel filtration column chromatography and found that the latter is the best. We selected the gel filtration column chromatography with Sepharose 4FF as the medium, and selected the method of sample volume, flow rate and other indicators, to determine the best method, and in the development of the purified vaccine hybrid protein Removal rate of 99 8% or more, for large-scale production provides a method.