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目的:用高亲和性μ阿片受体激动剂羟甲芬太尼研究小鼠脾脏淋巴细胞是否存在阿片受体。方法:观察羟甲芬太尼(Ohm)对脾脏淋巴细胞体外增殖的影响及纳络酮的阻断作用;采用[~3H]Ohm放射配体分析法检测小鼠脾脏淋巴细胞上的阿片受体。结果:Ohm 0.1pmol·L~(-1)-1nmol·L~(-1)增加Con A诱导的T-淋巴细胞增殖,这种增殖作用可以被纳络酮阻断。Ohm不能促进LPS诱导的B-细胞增殖。Ohm 0.1-1μmol·L~(-1)能够抑制活化的淋巴细胞增殖。这种抑制作用不被纳络酮阻断,[~3H]Ohm与淋巴细胞阿片受体的结合具有特异性、饱和性、可逆性。结论:Ohm对静止及活化的T-细胞的促增殖作用是由阿片受体介导的。小鼠脾脏淋巴细胞上存在阿片受体。
OBJECTIVE: To investigate the presence or absence of opioid receptors in splenic lymphocytes of mice by using high affinity μ opioid receptor agonist oxymetazidine. Methods: The effect of Ohm on proliferation of splenic lymphocytes in vitro and the blocking effect of naloxone were observed. [~ 3H] Ohm radioligand assay was used to detect the expression of opioid receptor . Results: Ohm 0.1 pmol·L -1 -1 nmol·L -1 increased Con A-induced T-lymphocyte proliferation, which could be blocked by naloxone. Ohm can not promote LPS-induced B-cell proliferation. Ohm 0.1-1μmol·L -1 can inhibit the activation of lymphocyte proliferation. This inhibitory effect is not blocked by naloxone, and the binding of [~ 3H] Ohm to lymphocyte opioid receptors is specific, saturable and reversible. CONCLUSIONS: The effect of Ohm on the proliferation of quiescent and activated T-cells is mediated by opioid receptors. Opioid receptors exist on mouse spleen lymphocytes.