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利用水稻秸秆模拟堆肥,对纤维素、半纤维素质量分数及堆肥相关指标随添加外源纤维素酶的变化进行研究。结果表明,与不加酶堆肥相比,添加外源纤维素酶显著提高了水稻秸秆纤维素及半纤维素的降解率,其降解趋势均可用一阶指数衰减方程拟合;堆肥结束时,添加纤维素酶处理的堆肥纤维素质量分数较不加酶处理低35.1%。纤维素酶促进了堆肥中腐殖酸的合成,提高了堆肥中的铵态氮(NO3-N)、硝态氮(NH4-N)及全氮(TN)的质量分数,并导致堆肥中碳素质量分数下降,C/N质量比下降了23.1%;添加纤维素酶进一步降低了堆肥的生物毒性,使得发芽指数提高了10.3%。外源添加纤维素酶加快了水稻秸秆堆肥的腐熟进程。
Rice straw was used to simulate compost, and the changes of cellulose, hemicellulose and compost related exogenous cellulase were studied. The results showed that compared with no enzyme compost, addition of exogenous cellulase significantly increased the degradation rate of rice straw cellulose and hemicellulose, the degradation trend can be fitted by first-order exponential decay equation; at the end of compost, add The cellulose content of cellulase treated composting was 35.1% lower than that of non-enzymatic treatment. The cellulase promoted the humic acid synthesis in composting, increased the mass fraction of ammonium nitrogen (NO3-N), nitrate nitrogen (NH4-N) and total nitrogen (TN) The mass fraction decreased and the mass ratio of C / N decreased by 23.1%. The addition of cellulase further reduced the biological toxicity of composting, resulting in an increase of germination index of 10.3%. Exogenous addition of cellulase accelerates the maturity of rice straw composting.