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目的:研究α-干扰素和维拉帕米对体外培养的乳腺癌细胞多药耐药的逆转作用。方法:MTT法检测各实验组细胞存活率,计算IC50、耐药倍数和逆转倍数;流式细胞术定量检测细胞表面P170的表达。结果:α-干扰素与维拉帕米联合应用使乳腺癌细胞耐阿霉素(ADM)的IC50降低为0.32μmolL-1,优于单用维拉帕米(1.23μmolL-1)、α-干扰素(2.29μmolL-1),逆转倍数由7.25和13.49提高到51.88;且P-170表达均有大幅度下降。结论:单独用α-干扰素、维拉帕米均可达到部分逆转乳腺癌耐药细胞(MCF-7/ADR)对ADM的耐药的作用,二者联合使用效果更强。
Objective: To study the reversal effects of α-interferon and verapamil on multidrug resistance in cultured breast cancer cells. Methods: The cell viability was measured by MTT assay, and the IC50, the multiple of drug resistance and the fold of multiple were calculated. The expression of P170 on the cell surface was quantitatively determined by flow cytometry. Results: The combination of α-interferon and verapamil reduced the IC50 of doxorubicin (ADM) in breast cancer cells to 0.32 μmol L-1, which was better than that of verapamil alone (1.23 μmol L -1) Interferon (2.29μmolL-1), fold increase from 7.25 and 13.49 to 51.88; and P-170 expression were significantly decreased. CONCLUSION: Both α-interferon alone and verapamil can partially reverse the drug resistance of breast cancer drug-resistant cells (MCF-7 / ADR) to ADM, and the combined effect is stronger.