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目的 为了解酪氨酸磷酸酶在造血细胞辐射损伤中的作用 ,深化对造血细胞辐射损伤分子机理的认识。方法 应用MTT法观察了酪氨酸磷酸酶抑制剂过钒酸钠对照射和未照射细胞增殖的影响。结果 过钒酸钠对细胞增殖的影响因培养基中G CSF浓度的不同而表现出相异的作用 :当G CSF浓度偏低时 ,一定浓度的过钒酸钠的加入可促进细胞增殖 ,而当G CSF浓度过高时 ,过钒酸钠的加入则抑制细胞生长 ;与未照射细胞相比 ,过钒酸钠对 3Gy照射细胞的促增殖作用明显加强 ,生长抑制作用相对减弱。结论 酪氨酸磷酸酶可能参与了细胞增殖的不同调控过程 ,而照射可能加强了细胞酪氨酸磷酸酶的增殖抑制作用。
Objective To understand the role of tyrosine phosphatase in the radiation injury of hematopoietic cells and to deepen the understanding of the molecular mechanism of radiation injury in hematopoietic cells. Methods The effect of tyrosine phosphatase inhibitor sodium vanadate on the proliferation of irradiated and non-irradiated cells was observed by MTT assay. Results The effect of sodium vanadate on cell proliferation showed different effects due to the different concentration of G CSF in the medium. When the concentration of G CSF was low, a certain concentration of sodium vanadate could promote cell proliferation, When G CSF concentration was too high, the addition of sodium vanadate inhibited the cell growth; compared with the non-irradiated cells, sodium orthovanadate on 3Gy irradiated cells significantly enhanced the role of promoting proliferation, growth inhibition was relatively weakened. Conclusion Tyrosine phosphatase may be involved in different regulation of cell proliferation, and irradiation may enhance the inhibition of cell tyrosine phosphatase proliferation.