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目的对吉祥草中总黄酮的体外抗氧化活性进行研究。方法采用盐酸-镁粉法测定黄酮含量,通过1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)法和铁离子还原(ferric ion antioxidant power,FRAP)法评价吉祥草总黄酮提取物的抗氧化活性。结果总黄酮提取物和脂溶性成分的EC50为(0.253±0.009)g·L-1,FRAP值为(0.964±0.028)mmol·g-1。结论吉祥草中总黄酮具有较强的抗氧化活性。
Objective To study the anti-oxidative activity of total flavonoids in jixiang grass. Methods The content of flavonoids was determined by hydrochloric acid-magnesium powder method. The content of flavonoids was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and ferric ion antioxidant power , FRAP) method to evaluate the antioxidant activity of total flavonoids extracted from jixiang grass. Results The EC50 of total flavonoids and fat-soluble components were (0.253 ± 0.009) g · L-1 and FRAP was (0.964 ± 0.028) mmol · g-1. Conclusion The total flavonoids in Ji Xiang Cao has strong antioxidant activity.