论文部分内容阅读
利用同源克隆的方法对11个立枯丝核菌标准融合群菌株的GPD基因进行了分离。分析发现不同融合群立枯丝核菌GPD基因在外显子数目、编码蛋白长度及内含子剪切方式等方面存在差异,如AG-2-2ⅢB、AG-2-2Ⅳ、AG-8三个融合群的GPD基因的外显子个数为10个,其他融合群均为11个;AG-8融合群的GPD基因的编码蛋白长度为267个氨基酸,其余融合群的GPD基因的编码蛋白长度约为340个氨基酸。AG-2-2ⅢB、AG-2-2Ⅳ、AG-8三个融合群的GPD基因在98、98、272氨基酸位点的内含子发生了缺失。基于蛋白序列的进化分析表明不同融合群之间GPD基因编码蛋白存在明显差异,可以有效地将不同融合群菌株区分开来;同时,不同物种之间GPD基因在进化上仍具有一定保守性,立枯丝核菌自身的GPD基因蛋白序列归为一类且与担子菌门同源性最高。这些结果表明进行保守基因序列差异分析是进行立枯丝核菌分类分群研究的新思路。
GPD genes of 11 Rhizoctonia solani standard fusion strains were isolated by homologous cloning method. The results showed that the GPD gene of Rhizoctonia solani in different fusion populations differed in number of exons, length of encoded protein and intron cleavage, such as AG-2-2ⅢB, AG-2-2Ⅳ and AG-8 The number of GPD genes in the fusion population was 10 and that in the other fusion populations was 11. The GPD gene of AG-8 fusion protein was 267 amino acids in length and the coding protein length of GPD genes in other fusion groups About 340 amino acids. The GPD gene of AG-2-2ⅢB, AG-2-2Ⅳ and AG-8 fusion was deleted in 98,98,272 amino acids. Phylogenetic analysis based on protein sequence showed that the GPD genes encoded by different fusion groups were significantly different and could effectively distinguish the different fusion strains. At the same time, GPD genes among different species were still conservative in evolution Rhizoctonia solani own GPD gene protein sequences classified as a class and Basidiomycotina homology highest. These results show that the analysis of conserved gene sequences is a new way to study Rhizoctonia solani taxonomy.