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目的观察紫外线照射对阴沟肠杆菌和表皮葡萄球菌生物学特性的影响。方法采用紫外线照射诱导,通过抑菌试验法和生化鉴定方法对诱变后细菌耐药性及生化特性进行了观察。结果阴沟肠杆菌和表皮葡萄球菌的原代菌耐受紫外线照射最长时间均为7 min,阴沟肠杆菌随着紫外照射的代数增多而存活率增加。经紫外线照射后的阴沟肠杆菌生化反应有改变,而表皮葡萄球菌生化反应无变化。经紫外线照射后的阴沟肠杆菌药物敏感变化较大,部分耐药性增加;而表皮葡萄球菌药物敏感变化较小。结论阴沟肠杆菌对紫外线的适应能力较表皮葡萄球菌强,紫外线照射可以诱导阴沟肠杆菌出现亚胺培南不稳定性耐药性增加。
Objective To observe the effects of ultraviolet radiation on the biological characteristics of Enterobacter cloacae and Staphylococcus epidermidis. Methods UV irradiation was used to induce bacterial resistance and biochemical characteristics after antibacterial test and biochemical identification. Results The primary bacteria of Enterobacter cloacae and Staphylococcus epidermidis were exposed to ultraviolet light for a maximum of 7 min. The viability of Enterobacter cloacae increased with the generation of ultraviolet radiation. After the UV irradiation of Enterobacter cloacae biochemical reactions have changed, while Staphylococcus epidermidis biochemical reactions did not change. After the UV irradiation of Enterobacter cloacae drug sensitive changes in some of the increased resistance; and Staphylococcus epidermidis drug-sensitive changes in small. Conclusion The ability of Enterobacter cloacae to adapt to ultraviolet light is stronger than that of Staphylococcus epidermidis. Ultraviolet irradiation can induce the increase of instability of imipenem in Enterobacter cloacae.