论文部分内容阅读
目的 研究焦点粘附激酶 (FAK )在肝细胞癌 (HCC)中的表达、活化及其临床意义。方法 采用原位分子杂交法检测 3 5例肝细胞癌 ,8例肝硬变及 5例正常肝组织中FAKmRNA的表达 ,并通过Western印迹杂交法分析肝癌组织中FAK的磷酸化 (Tyr -3 97)状态。 结果 肝癌组织中FAKmRNA水平明显高于肝硬变、正常肝组织 ( P <0 .0 1) ;同时低分化、存在转移的肝癌组织中FAK磷酸化水平明显高于高分化、无转移的肝癌 (P <0 .0 5 )。结论 FAK的高表达及磷酸化水平的上调可能参与了肝癌的发生发展 ,检测FAK的磷酸化 (Tyr -3 97)水平具有一定的临床病理意义
Objective To study the expression and activation of focal adhesion kinase (FAK) in hepatocellular carcinoma (HCC) and its clinical significance. Methods The expression of FAK mRNA in 35 hepatocellular carcinomas, 8 cirrhosis and 5 normal liver tissues was detected by in situ hybridization. The phosphorylation of FAK was analyzed by Western blotting (Tyr -3 97 )status. Results The level of FAK mRNA in HCC tissues was significantly higher than that in cirrhosis and normal liver tissues (P <0.01). Meanwhile, the level of FAK phosphorylation in HCC tissues with poorly differentiated and metastatic tumors was significantly higher than that in well-differentiated and metastatic HCC P <0. 05). Conclusion The high expression of FAK and the up-regulation of phosphorylation may be involved in the development of hepatocellular carcinoma. The detection of phosphorylation of Tyr-3 (97) in FAK has some clinicopathological significance