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Human induced pluripotent stem cells(iPSCs)hold great promise for regenerative medicine.Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells(UCBMCs)is of great signifcance.Here we report generation of human iPSCs with great effciency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors.We generated these cells by optimizing the existing iPSC induction protocol.The UCBMC-derived iPSCs(UCB-iPSCs)have characteristics that are identical to pluripotent human embryonic stem cells(hESCs).This study highlights the use of UCBMCs to generate highly functional human iPSCs that could accelerate the development of cell-based regenerative therapy for patients suffering from various diseases.
Human induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononuclear cells (UCBMCs) is of great signifance. Here we report generation of human iPSCs with great effciency from UCBMCs using a dox- inducible lentiviral system carrying four Yamanaka factors. We generated these cells by optimizing the existing iPSC induction protocol. UCBMC-derived iPSCs (UCB-iPSCs) have characteristics that are identical to pluripotent human embryonic stem cells (hESCs). This study highlights the use of UCBMCs to generate highly functional human iPSCs that could accelerate the development of cell-based regenerative therapy for patients suffering from various diseases.