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目的从细胞分子水平研究电压门控型钾通道亚型Kv1.2、Kv3.4在15-酮基二十碳四烯酸(15-ketoeicosatetretraenoic acid,15-KETE)致大鼠肺动脉收缩过程中的作用。方法通过酶法分离、培养SD大鼠肺动脉平滑肌细胞(pulmonary arterial smooth cells,PASMCs),使用RT-PCR和Western blot技术观察15-KETE对大鼠PASMCs上Kv1.2、Kv3.4通道表达的影响。结果 (1)15-KETE下调大鼠PASMCs膜上Kv1.2通道mRNA及蛋白质的表达;(2)15-KETE下调大鼠PASMCs膜上Kv3.4通道mRNA及蛋白质的表达。结论 Kv1.2、Kv3.4通道参与由15-KETE引起的缺氧肺动脉收缩(hypoxic pulmonary vasoconstriction,HPV)。
Objective To study the effects of Kv1.2 and Kv3.4, a type of voltage-gated potassium channel, on the contraction of rat pulmonary arteries induced by 15-ketoeicosatetretraenoic acid (15-KETE) at the cellular and molecular level effect. Methods Pulmonary artery smooth muscle cells (PASMCs) of SD rats were isolated and cultured by enzymatic method. The effects of 15-KETE on the expression of Kv1.2 and Kv3.4 channels in PASMCs were observed by RT-PCR and Western blot . Results (1) 15-KETE down-regulated the mRNA and protein expression of Kv1.2 channel in rat PASMCs. (2) 15-KETE down-regulated the mRNA and protein expression of Kv3.4 channel in PASMCs. Conclusion Kv1.2 and Kv3.4 channels are involved in hypoxic pulmonary vasoconstriction (HPV) induced by 15-KETE.