对２４例酶联免疫吸附试验（ＥＬＩＳＡ）ＨＢｓＡｇ阴性的原发性胆管细胞性肝癌的石蜡包埋肝组织，应用ＰＣＲ－ＥＢ法检测了乙型肝炎病毒（ＨＢＶ）ＤＮＡ，并与ＥＬＩＳＡ法检测的血清免疫学标志物（乙肝５项：ＨＢｓＡｇ、抗－ＨＢｓ、ＨＢｅＡｇ、抗－ＨＢｅ、抗－ＨＢｃ）结果进行对比。结果显示血清学乙肝５项均阴性的及ＨＢｓＡｇ阴性但其它４项中至少有１项阳性的胆管细胞性肝癌石蜡包埋肝组织中，ＨＢＶＤＮＡ阳性检出率分别为３８．９％和４５．８％，证实ＨＢｓＡｇ血清学阴性者肝组织中仍有ＨＢＶＤＮＡ存在 Twenty-four cases of hepatitis B virus (HBV) DNA were detected by PCR-EB method in 24 paraffin-embedded liver tissues of HBsAg-negative cholangiocarcinoma with ELISA, Serum immunological markers (hepatitis B 5: HBsAg, anti-HBs, HBeAg, anti-HBe, anti-HBc) were compared. The results showed that the positive rate of HBVDNA was 5% in seropositive HBsAg-positive and 5-serogroup HBsAg-positive in cholangiocarcinoma of the cholangiocarcinoma of the cholangiocarcinoma with 5 negative HBsAg but at least 1 positive of the other 4 %, Confirming the presence of HBVDNA in the liver tissue of HBsAg seronegative patients