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在自组装膜修饰的硅表面制备有序的蛋白阵列是研发生物传感器的先决条件之一,因此如何产生有序的表面蛋白阵列一直是生物医药研究方向的前沿。本研究通过应用纳米球刻蚀法在氧化的10-烯基十一烷基三氯硅烷自组装膜修饰的硅表面生成了网状结构溶菌酶蛋白层。网孔的大小(从纳米到微米级别)由表面沉积的纳米球的尺寸来调控。我们利用原子力显微镜和荧光显微镜对样品表面进行了详细表征。结果表明:这种新方法比传统的通过扫描探针在固体表面修饰而聚集溶菌酶蛋白的方法更快捷简便,而且它能够在相对大的硅表面形成网状蛋白层。此外,网孔表面附着具有强吸附活性的羧酸基团层,它可以通过静电吸引或者共价结合来吸附液相中的第二种蛋白分子。
It is one of the preconditions for biosensor to prepare ordered protein arrays on self-assembled monolayers. Therefore, how to generate ordered surface protein arrays has always been the frontier of biomedical research. In this study, the network structure of lysozyme protein layer was formed by the nanosphere etching method on the surface of silicon modified by oxidized 10-alkenyl undecyltrichlorosilane self-assembled film. The size of the meshes (from nanometers to micrometers) is governed by the size of the surface-deposited nanospheres. We used atomic force microscopy and fluorescence microscopy to characterize the sample surface. The results show that this new method is faster and easier than the traditional method of aggregating lysozyme protein on solid surface by scanning probe, and it can form reticular protein layer on relatively large silicon surface. In addition, the surface of the mesh is attached with a strongly adsorbed carboxylic acid group, which adsorbs the second protein molecule in the liquid phase by electrostatic attraction or covalent bonding.