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目的:探讨高特异性HER2/CEP17双色探针在浸润性乳腺癌中的诊断价值。方法:制备高特异性HER2/CEP17双色探针,通过外周血细胞核型滴片检测该探针的特异性,同时用传统探针和新制备探针对425例浸润性乳腺癌患者进行FISH检测,并对结果进行对比分析,评估高特异性HER2/CEP17探针在浸润性乳腺癌中的临床诊断价值。结果:染色体定位显示,传统的CEP17探针在17号染色体着丝粒处杂交信号明亮,在Y染色体着丝粒处可见一个较17号染色体略弱但肉眼可辨的杂交信号点;而新制备CEP17探针在17号染色体着丝粒处具有明亮的杂交信号,在其余染色体中未见明显的杂交信号点,表明该探针具有较高的特异性。临床样本检测中,新制备探针与传统探针对浸润性乳腺癌HER2基因扩增的检测无统计学差异(P=0.500,χ~2=0.5),然而,对于CEP17杂信号,统计结果表明新制备探针明显少于传统探针(P=0.013,χ~2=5.29)。结论:新方法制备HER2/CEP17探针具有较高的特异性,相比于传统探针更具有临床应用优势。
Objective: To investigate the diagnostic value of high specificity HER2 / CEP17 two-color probe in invasive breast cancer. Methods: The high-specificity HER2 / CEP17 two-color probe was prepared and the specificity of the probe was detected by peripheral blood cell karyotype. 425 cases of invasive breast cancer were detected by FISH with traditional probe and newly prepared probe, The results were compared and analyzed to evaluate the clinical diagnostic value of high specific HER2 / CEP17 probe in invasive breast cancer. Results: Chromosomal mapping showed that the CEP17 probe hybridized brightly at the centromere of chromosome 17 and showed a slightly weaker, but discernable hybridization signal point on the centromere of chromosome Y. The newly prepared The CEP17 probe has a bright hybridization signal at the centromere of chromosome 17 and no obvious hybridization signal points in the other chromosomes, indicating that the probe has high specificity. There was no significant difference in the amplification of HER2 gene between invasive and invasive breast cancer (P = 0.500, χ ~ 2 = 0.5). However, for the CEP17 heterozygote, the statistical results showed that The number of newly prepared probes was significantly less than that of the conventional probes (P = 0.013, χ ~ 2 = 5.29). Conclusion: The new method of preparation of HER2 / CEP17 probe has high specificity, compared with the traditional probe more clinical application advantages.