作者总结大鼠纖维母细胞分为3組用纖維素凝块培养基体外培养。第1组加甲基胆蒽,第2組加甲基胆蒽及小鼠乳腺瘤抽出液,第3組无处理以作对照。每组之細胞任培养基上作单层的生长。至6个月上下将各組分别作成悬液而接种于初生之大鼠。結果所有3組接种后,皆发展成肉瘤。于是証实大鼠纖維母细胞在体外培养基上經过长期培养,确能造成自发恶性变化。 The authors concluded that rat fibroblasts were divided into 3 groups and cultured in vitro with cellulose clot medium. Group 1 plus methyl-cholesterol, group 2 plus methyl-cholesterol and mouse mammary aspirate fluid, group 3 without treatment as a control. The cells of each group were grown as a monolayer on the medium. Each group was made into a suspension for up to 6 months and inoculated into a primary rat. Results After all three groups were inoculated, all developed into sarcomas. Thus, it was confirmed that the rat fibroblasts were cultured in vitro for a long period of time and indeed caused spontaneous malignant changes.